Molecular detection of murine gammaherpesvirus 68 (MHV-68) in Haemaphysalis concinna ticks collected in Slovakia

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Murine gammaherpesvirus (MHV-68) transforms cultured cells in vitro.

Human dermal fibroblasts and mouse NIH/3T3 cells acquired the transformed phenotype ('criss-cross' pattern of growth) after infection with ultraviolet-irradiated murine gammaherpesvirus (MuHV-4 strain 68; MHV-68). These cells with changed phenotype could be serially cultured for 5-6 passages (35-40 days), and then they entered into crisis and most of them died. In a small number of cultures, ho...

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Murine Gammaherpesvirus (MHV-68) Transforms Cultured Cells in vitro

Human dermal fibroblasts and mouse NIH/3T3 cells acquired the transformed phenotype (‘criss-cross’ pattern of growth) after infection with ultraviolet-irradiated murine gammaherpesvirus (MuHV-4 strain 68; MHV-68). These cells with changed phenotype could be serially cultured for 5–6 passages (35–40 days), and then they entered into crisis and most of them died. In a small number of cultures, ho...

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Detection of Transcripts and an Infectious Dose of Murine Gammaherpesvirus 68 in Dermacentor reticulatus Ticks

Background: Murine gammaherpesvirus 68 is assumed to be a natural pathogen of murid rodents. Previous investigations of MHV68 in field-collected Dermacentor reticulatus, Ixodes ricinus, and Haemaphysalis concinna ticks support the idea that ticks acquire the virus from feeding on infected hosts. Based on our previous finding of a live MHV-68 capable to replicate in mammalian cells, we aimed to ...

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Glycosaminoglycan Interactions in Murine Gammaherpesvirus-68 Infection

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Herpesviruses are a large group of DNA viruses infecting mainly vertebrates. Murine gammaherpesvirus 68 (MHV68) is often used as a model in studies of the pathogenesis of clinically important human gammaherpesviruses such as Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus. This rodent virus appears to be geographically widespread; however, its natural transmission cycle is unknow...

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ژورنال

عنوان ژورنال: Acta virologica

سال: 2016

ISSN: 1336-2305

DOI: 10.4149/av_2016_04_426